Antigen families

DC cell subtype


Dendritic cells (DCs) do not represent a single uniform population, but rather display a considerable degree of heterogeneity (1,2). One of the challenges in DC research and use of these cells as therapeutic targets will be to better understand the specific biology associated with the different DC subtypes.
Subtypes of DCs can be identified according to their localization, phenotype, and immune function. Langerhans cells represent the DCs of epidermis and stratified mucosal epithelia (3) and are characterized by their expression of Langerin/CD207 (mAb DENDRITICS) (4). The class 6 semaphorin SEMA 6A (mAb DENDRITICS: DDX0462, DDX0463)) further defines an activation status of Langerhans cells (5). DCs of the dermis or the lamina propria of mucosae (interstitial DCs) constitute more diverse populations, characterized by the expression of DC-SIGN/ CD209 (DC-SIGN-like, mAb DENDRITICS) in humans. Positive identification of such DCs is comforted by other markers such as DEC205/ CD205 (mAb DENDRITICS), CD1a (mAb DENDRITICS: DDX0080, DDX0081) (in humans only) and the coagulation factor FXIIIa (6). Other molecules expressed by interstitial DCs, or subsets thereof, include the macrophage mannose receptor (MMR/CD206) MMR (mAb DENDRITICS: DDX0380, DDX0381) and the macrophage galactose lectin/ asialoglycoprotein receptor/CD301 (mAb DENDRITICS: DDX0010, DDX0011) (7), DCIR (8) (mAb DENDRITICS), and FDF03 (9) (mAb DENDRITICS). Many features of interstitial DCs are shared by macrophages, a fact that illustrates the existence of a broad continuum between these two cell types. Both Langerhans cells and interstitial DCs are highly specialized in the capture (internalization/endocytosis) of exogenous antigens through various mechanisms. Following appropriate activation signals, cutaneous and mucosal DCs undergo a maturation sequence and migrate via lymphatic vessels into draining lymphoid tissue, where they can efficiently present processed antigen to specific T lymphocytes (10). However, it is unclear if different DCs that reach lymphoid tissue will intrinsically imprint their signature to the type or intensity of the immune response. Likewise, the comparative potential of these DC subtypes to induce a state of immune tolerance remains to be clarified. Interdigitating DCs, localized in the T cell areas of secondary lymphoid tissue, express the lysosome-associated marker DC-LAMP (11) (mAb DENDRITICS), as well as the costimulatory molecules CD40 (mAb DENDRITICS: DDX0130, DDX0131), CD80, and CD54/ICAM1 (mAb DENDRITICS: DDX0150, DDX0151). By contrast, molecules such as the Ig superfamily member DORA (mAb DENDRITICS) are downregulated upon DC activation (12) . A relatively recent, but highly important addition to the DC family is the plasmacytoid DC (pDC), also known as natural Interferon-Producing Cell (IPC) (13, 14). pDCs differ from the «conventional» DCs described above by a number of criteria. Phenotypically, pDC can be identified by their expression of CD303 (mAb DENDRITICS), IL-3 receptor alpha chain/CD123 (mAb DENDRITICS), and lack of CD11c, a feature of most other DCs. Neuropilin-1/CD304 (mAb DENDRITICS) is also expressed by pDCs, although not entirely restricted to this DC subtype. Mouse pDCs/IPCs display a cell-surface molecule recognized by monoclonal antibody 120G8/BST-2/CD317 (mAb DENDRITICS) which is a useful tool to deplete this DC subtype in vivo. Functionally, pDCs are characterized by their capacity to produce high amounts of type-I interferons (IFN-α/β) upon viral stimulation. Unlike conventional DCs which produce IL-12, pDCs have modest antigen-presentation function, their role being rather to link innate and adaptive immunity. In steady-state, pDCs occur in peripheral blood and lymphoid tissue, into which they immigrate via high endothelial venules. pDC undergo further maturation in response to IL-3 plus CD40-ligand/CD154 (mAb DENDRITICS: DDX0140, DDX0141) (13), or to the cytokines IFN-α or TNF-α. (15). DC subtypes found in the thymus differ from those in lymph nodes or spleen. Thymic DCs do however express markers such as Langerin/CD207 (mAb DENDRITICS) (16) and DEC205/CD205 (mAb DENDRITICS), that are associated with other DC subtypes. Thymic DCs are involved in shaping central tolerance of the developing T cell repertoire. (17)  Finally, follicular DCs, expressing the long-isoform of CR2/ CD21 (mAb DENDRITICS) (18), represent a cell-type ontogenically distinct from all other DCs, and found within B lymphocyte follicles of secondary lymphoid tissue.

References
1. Banchereau J., Steinman R.M.: Dendritic cells and the control of immunity. 1998 Nature 392: 245-252
2. Shortman K.,and Liu YJ., Mouse and human DC subtypes 2002 Nature Rev Immunol 2: 151-161
3. Romani N, Holzmann S, Tripp CH, Koch F, Stoitzner P.: Langerhans cells – dendritic cells of the epidermis. 2003 APMIS 111: 725-740
4. Valladeau J, Ravel O, Dezutter-Dambuyant C, Moore K, Kleijmeer M, Liu Y, Duvert-Frances V, Vincent C, Schmitt D, Davoust J, Caux C, Lebecque S, Saeland S., Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules», 2000 Immunity 12: 71-81
5. Gautier G, de Saint-Vis B, Senechal B, Pin JJ, Bates EE, Caux C, Geissmann F, Garrone P. The class 6 semaphorin SEMA6A is induced by interferon-gamma and defines an activation status of langerhans cells observed in pathological situations, 2006 Am J Pathol 168: 453-465
6. Valladeau J, Saeland S.: Cutaneous dendritic cells.,2005 Seminars Immunol 17: 273-283
7. Valladeau J, Duvert-Frances V, Pin JJ, Kleijmeer MJ, Ait-Yahia S, Ravel O, Vincent C, Vega F Jr, Helms A, Gorman D, Zurawski SM, Zurawski G, Ford J, Saeland S. Immature human dendritic cells express asialoglycoprotein receptor isoforms for efficient receptor-mediated endocytosis., 2001 J Immunol 167: 5767-5774
8. Bates EE, Fournier N, Garcia E, Valladeau J, Durand I, Pin JJ, Zurawski SM, Patel S, Abrams JS, Lebecque S, Garrone P, Saeland S. APCs express DCIR, a novel C-type lectin surface receptor containing an immunoreceptor tyrosine-based inhibitory motif., 1999, J. Immunol 163: 1973-1983
9. Fournier N, Chalus L, Durand I, Garcia E, Pin JJ, Churakova T, Patel S, Zlot C, Gorman D, Zurawski S, Abrams J, Bates EE, Garrone P., FDF03, a novel inhibitory receptor of the immunoglobulin superfamily, is expressed by human dendritic and myeloid cells 2000 J Immunol 165: 1197-1209
10. Guermonprez P, Valladeau J, Zitvogel L, Thery C, Amigorena S., Antigen presentation and T cell stimulation by dendritic cells, 2002 Ann Rev Immunol 20: 621-667
11. de Saint-Vis B, Vincent J, Vandenabeele S, Vanbervliet B, Pin JJ, Ait-Yahia S, Patel S, Mattei MG, Banchereau J, Zurawski S, Davoust J, Caux C, Lebecque S. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment, 1998 Immunity 9: 325-336
12. Bates EE, Dieu MC, Ravel O, Zurawski SM, Patel S, Bridon JM, Ait-Yahia S, Vega F Jr, Banchereau J, Lebecque S., CD40L activation of dendritic cells down-regulates DORA, a novel member of the immunoglobulin superfamily 1998 Mol Immunol 35: 513-524
13. Grouard G, Rissoan MC, Filgueira L, Durand I, Banchereau J, Liu YJ., The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligan, 1997 J Exp Med 185: 1101-1111
14. Liu YJ., IPC: professional type 1 interferon-producing cells and plasmacytoid dendritic precursors 2005 Ann Rev Immunol 23: 275-306
15. Kadowaki N, Antonenko S, Lau JY, Liu YJ.« Natural interferon alpha/beta-producing cells link innate and adaptive immunity, J Exp Med. 2000 192(2):219-26
16. Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. 2002, J Immunol, 168: 782-792
17. Wu L, Shortman K., Heterogeneity of thymic dendritic cells », 2005 Semin Immunol 17 : 304-312
18. Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW., Follicular dendritic cells specifically express the long CR2/CD21 isoform 1997 J Exp Med 185 : 165-170

Click on an antigen to find the related products.

  • ● CD303

    • No available product.
  • ● ASGPR/CD301

    • DDX0010 - 125A10.03
      DDX0010 125A10.03
      ASGPR/CD301
      Mouse IgG1

      Internalization

    • DDX0011 - 121A5.01
      DDX0011 121A5.01
      ASGPR/CD301
      Mouse IgG1

      Internalization

  • ● CD1a

    • DDX0080 - 214A9.01
      DDX0080 214A9.01
      CD1a
      Mouse IgM
    • DDX0081 - 201B5.08
      DDX0081 201B5.08
      CD1a
      Mouse IgG1
  • ● Mouse CD8 alpha

    • DDX0091 - 53.6.72 (Lyt-2)
      DDX0091 53.6.72 (Lyt-2)
      Mouse CD8 alpha
      Rat IgG2a
  • ● CD40

    • DDX0130 - G28-5
      DDX0130 G28-5
      CD40
      Mouse IgG1k

      IHC for paraffin-embedded tissues

    • DDX0131 - mAb89
      DDX0131 mAb89
      CD40
      Mouse IgG1

      B cell activation, IHC for paraffin-embedded tissues

  • ● Langerin-associated lectin/Human Kupffer cell receptor homolog

    • DDX0350 - 109B8.02
      DDX0350 109B8.02
      Langerin-associated lectin/Human Kupffer cell receptor homolog
      Mouse IgG1

      Küpffer cell receptor

    • DDX0351 - 117E10.10
      DDX0351 117E10.10
      Langerin-associated lectin/Human Kupffer cell receptor homolog
      Mouse IgG1

      Küpffer cell receptor

  • ● DCIR/CD367

    • DDX0180 - 111F8.04
      DDX0180 111F8.04
      DCIR/CD367
      Mouse IgG1
    • DDX0181 - 108H8.03
      DDX0181 108H8.03
      DCIR/CD367
      Mouse IgG1
  • ● DC-LAMP/CD208

    • DDX0190 - 104G4
      DDX0190 104G4
      DC-LAMP/CD208
      Mouse IgG1
    • DDX0191 - 1010E1.01
      DDX0191 1010E1.01
      DC-LAMP/CD208
      Rat IgG2a

      IHC for paraffin-embedded tissues; multi-species cross reactivity

    • DDX0192 - 1006F7.05
      DDX0192 1006F7.05
      DC-LAMP/CD208
      Rat IgG2a

      Murine DC-LAMP

    • DDX0193 - 109G3
      DDX0193 109G3
      DC-LAMP/CD208
      Mouse IgG1
    • DDX0195 - 208B5
      DDX0195 208B5
      DC-LAMP/CD208
      Mouse IgG3

      Multi-species cross reactivity

  • ● DC-SIGN/CD209

    • DDX0202 - 102E11.06
      DDX0202 102E11.06
      DC-SIGN/CD209
      Mouse IgG2b,k

      IHC for paraffin-embedded tissues

    • DDX0203 - 103G2.07
      DDX0203 103G2.07
      DC-SIGN/CD209
      Mouse IgG1k

      IHC for paraffin-embedded tissues

    • DDX0204 - 108C7.01
      DDX0204 108C7.01
      DC-SIGN/CD209
      Mouse IgG2b,k

      HIV gp120 binding studies

    • DDX0205 - 102F10.04
      DDX0205 102F10.04
      DC-SIGN/CD209
      Mouse IgG2b,k

      Internalization

    • DDX0208 - 120C11.01
      DDX0208 120C11.01
      DC-SIGN/CD209
      Mouse IgG2b,k

      Inhibits HIV gp120 binding to DC-SIGN

    • DDX0207 - 109H12.03
      DDX0207 109H12.03
      DC-SIGN/CD209
      Mouse IgG2a
    • DDX0209 - 111H2.02
      DDX0209 111H2.02
      DC-SIGN/CD209
      Mouse IgG2b

      IHC for paraffin-embedded tissues

  • ● Dec205/CD205 (NLDC145)

    • DDX0020 - NLDC-145
      DDX0020 NLDC-145
      Dec205/CD205 (NLDC145)
      Rat IgG2a
  • ● DORA/IgSF6

    • DDX0220 - 104A10.01
      DDX0220 104A10.01
      DORA/IgSF6
      Mouse IgG1
  • ● FDFO3

    • DDX0230 - 36H2
      DDX0230 36H2
      FDFO3
      Rat IgG2a
  • ● IL3R Alpha/ CD123

    • DDX0300 - 107D2.08
      DDX0300 107D2.08
      IL3R Alpha/ CD123
      Mouse IgG1

      IHC for paraffin-embedded tissues

    • DDX0301 - 106C2.02
      DDX0301 106C2.02
      IL3R Alpha/ CD123
      Mouse IgG1

      Cross-reactivity with porcine cells

  • ● Langerin/CD207

    • DDX0360 - 808E10.01
      DDX0360 808E10.01
      Langerin/CD207
      Mouse IgG1

      Extracellular epitope (CRD)

    • DDX0361 - 310F7.02
      DDX0361 310F7.02
      Langerin/CD207
      Mouse IgG1

      Multi-species cross reactivity , Intracellular epitope, IHC for paraffin-embedded tissues

    • DDX0362 - 929F3.01
      DDX0362 929F3.01
      Langerin/CD207
      Rat IgG2a

      Multi-species cross reactivity, Extracellular epitope (neck domain) , IHC for paraffin-embedded tissues

    • DDX0363 - DCGM4/122D5
      DDX0363 DCGM4/122D5
      Langerin/CD207
      Mouse IgG1

      Extracellular epitope (CRD)

    • DDX0368 - 306G9
      DDX0368 306G9
      Langerin/CD207
      Mouse IgG1

      Intracellular epitope ; IHC for paraffin-embedded tissues

    • DDX0370 - 205C1
      DDX0370 205C1
      Langerin/CD207
      Mouse IgM

      Murine langerin extracellular domain (CRD)

    • DDX0373 - 923B7
      DDX0373 923B7
      Langerin/CD207
      Rat IgG2a

      Extracellular epitope (neck domain)

    • DDX0376 - 918A11
      DDX0376 918A11
      Langerin/CD207
      Rat IgG2a

      Extracellular epitope (neck domain)

    • DDX0377 - 817G7
      DDX0377 817G7
      Langerin/CD207
      Mouse IgG1

      Extracellular epitope (CRD)

  • ● Mannose Receptor/CD206

    • DDX0380 - 122D2.08
      DDX0380 122D2.08
      Mannose Receptor/CD206
      Mouse IgG1

      Extracellular domain

  • ● Mouse DCS to be characterized

    • No available product.
  • ● Neuropilin/CD304

    • DDX0440 - 211H6.01
      DDX0440 211H6.01
      Neuropilin/CD304
      Mouse IgG1

      Extracellular domain, specific of PDC and DC neuropilin isoform

  • ● pDC/IPC/BST-2/CD317

    • DDX0390 - 120G8.04
      DDX0390 120G8.04
      pDC/IPC/BST-2/CD317
      Rat IgG1k

      In vivo depletion of murine PDC

  • ● Sema-6A

    • DDX0462 - 104B3
      DDX0462 104B3
      Sema-6A
      Mouse IgG1

      Extracellular epitope, specifically stains Langerhans histiocytosis samples

    • DDX0463 - 118E7
      DDX0463 118E7
      Sema-6A
      Mouse IgG1

      Extracellular epitope, stains activated Langerhans cells